植物病害分子流行学导论

出版时间：2007-9  出版社：中国农业大学出版社  作者：骆勇，马忠华，马占鸿　著  页数：208  字数：340000  

内容概要

　　《植物病害分子流行学导论（英文版）》主要内容包括：Protein—based marker：Allozymes、DNA band—based markers、DNA sequence、References and suggested readings、CltAPTER 3 PCR—BASED TECHNIQUES、Classical PCR、Allele—specific PCR、Nested PCR、Multiplex PCR等等。

书籍目录

PART I  INTRoDUCTION　CHAPTER 1  INTRoDUCl’ION TO MoLECULAR EPIDEMIOLoGY　　1—1 Basic concept of molecular epidemiology　　1—2 Why molecular epidemiology　　1—3 Comparisons between classical and molecular methods in epidemiology　　1—4 Working approaches of molecular epidemiology　　1—5 About the book　　References and suggested readingsPARTⅡMoLECULAR TECHNIQUES USED IN EPIDEMIOLOGY　CHAPTER 2 MOLECULAR MARKERS　　2—1 Protein—based marker：Allozymes　　2—2  DNA band—based markers　　2—3  DNA sequence　　References and suggested readings　CltAPTER 3 PCR—BASED TECHNIQUES　　3—1  Classical PCR　　3—2  Allele—specific PCR　　3—3  Nested PCR　　3—4  Multiplex PCR　　References and suggested readings　CHAPTER 4 QUANTITATIVE REAL‘TIME PCR　　4—1  Introduction　　4—2  Basic real—time PCR methods　　4—3 Primer and probe design for real—time PCR　　4—4  Result analysis　　4—5  Applications of real—time PCR　　References and suggested readings　CHAmR 5  PRIMER I)ESIGN　　5—1  Some basic concepts for primer design　　5—2 Computer programs used for primer design　　5—3  An example of primer design using a computer program　　5—4 Degenerated PCR primersPARTⅢ  MoLECULAR EPIDEMIOLOGY oF PLANT DISEASES　CHAPTER 6 PATHoGEN IDENTIFICATIoN AND DISEASE DIAGNoSIS　　6—1  Introduction　　6—2 Use of species—specific genes or DNA regions for developing PCR primers　　6—3 Use of anonymous unique DNA regions for developing PCR primers　　6—4 PCR inhibitors　　Summary　　References and suggested readings　CHAPTER 7 FUNGAL PATHoGEN REPRoDUCTIVE MoDES　　7—1  Frequencies of genotypes　　7—2 Deviation from Hardy—Weinberg expectations　　7—3 Parsimony Tree Length Permutation(PTLPT)　　7—4  Index of association(IA)　　References and suggested readings　CHAPTER 8  DETEMINATIoN AND QUANTIFICATION OF INITIAL INOCULUM OF EPIDEMICS　　8—1  Introduction　　8—2  Molecular methods for quantification of initial inoculum　　8—3 Examples of quantification of initial inoculum using molecular methods　　Remarkable conclusion　　References and suggested readings　CHPTER 9 TEMpORAL AND SPATIAL DEVELOPMENT oF PATHoGEN POPULATIoNS　　9—1  Introduction　　9—2  Molecular approaches　　9—3  Monitoring pathogen population structures　　9—4 Studying spatial—temporal dynamics of pathogen populations　　9—5 Providing information needed for disease management　　Summary　　References and suggested readings　CHAPTER 10  PATHoGEN LONG—DISTANCE DIsPERSAL AND MIGRATION　　10—1  Introduction　　10—2 Pathogen lon9—distance dispersal and disease epidemics　　10—3 Traditional methods to determine or infer pathogen lon9—distance dispersal　　10—4 Molecular methods to study pathogen lon9—distance dispersal　　10一5  Examples　　Summary　　……PART IV METHODOLOGY OF DATA ANLYSISINDEX