出版时间:2008-10 出版社:西南师范大学出版社 作者:夏庆友,向仲怀 编 页数:651
内容概要
这本研究论文集总结8年来中国家蚕基因组计划的主要研究结果,为已经启动并有着美好前景的家蚕功能基因组计划提供借鉴和参考。全书分为四个部分,收录了这个期间正式发表的论文共计68篇,分别反映中国家蚕基因组计划之背景、意义和策略(4篇);测序、生物信息学和基因组分析(15篇);关键平台技术(17篇)和功能基因组及其应用(31篇)等。
书籍目录
家蚕基因组研究对蚕业学科和产业发展的影响(代序)前言第一部分 背景、意义和策略21世纪的丝绸之路国际鳞翅目昆虫基因组筑波会议与中国家蚕基因组为丝绸之路重添异彩关于尽快启动“中国家蚕基因组计划”的建议中国家蚕基因组与21世纪丝绸之路第二部分 测序、生物信息学和基因组分析A draft sequence for the genome of the domesticated silkworm (Bombyw mori)家蚕(Bombyx mori ) 全基因组框架图SilkDB:a knowledgebase for silkworm biology and genomicsEST-based profiling and comparison of gene expression in the silkworm fat body during metamorphosisMining single nucleotide polymorphisms from EST data of silkworm, Bombyx mori , inbred strain DazaoDetection and analysis of alternative splicing in the silkworm by aligning expressed sequence tags with the genomic sequenceAnalysis on frequency and density of microsatellites in coding sequences of several eukaryotic genomesSurvey of long terminal repeat retrotransposons of domesticated silkworm(Bombyx mori )家蚕cDNA文库构建及大规模EST测序家蚕母性基因的表达序列标签分析家蚕chi、gluE和fruA基因与微生物相应基因的同源性及基因水平转移初探家蚕细胞色素P450的基因组学分析家蚕脂肪体组织基因表达谱的研究工.家蚕5龄中期幼虫脂肪体组织基因表达分析家蚕线粒体基因组1.8kb片段的克隆及序列分析家蚕线粒体基因组全序列测定与分析第三部分 关键平台技术Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx moriIdentification and characterization of piggyBac-like elements in the genome of domesticated silkworm, Bombyx moriStudies on middle and posterior silk glands of silkworm (Bombyx mori) using two-dimensional electrophoresis and mass spectrometryEstablishment and characterization of two embryonic cell lines of Bombyx mori利用精子介导法向蚕卵导人外源基因的研究家蚕转基因载体pBacA3EG的构建及其表达GAL4/UAS系统在转基因技术中的应用研究进展红色荧光标记载体pBacA4DsRed的构建及其在蚕卵中的瞬时表达家蚕转基因载体pBacA3EG在家蚕培养细胞中的表达家蚕丝腺蛋白质组学研究方法的建立家蚕中肠组织蛋白质组学研究家蚕胚胎发育时期的RNA干涉研究一种家蚕卵巢原代细胞制作方法探索家蚕胚胎细胞系的DNA指纹图谱分析家蚕胚胎细胞系BmE—SWU2的建立及其生物学特性研究家蚕脂肪体组织原代培养及其细胞分化的研究CELI酶的粗纯化及活性分析第四部分 功能基因组及其应用Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx moriProteolytic activation of pro-spfitzle is required for the induced transcription of antimicrobial peptide genes in lepidopteran insectsIdentification and analysis of Toll-related genes in the domesticated silkworm, Bombyx moriCharacterization and expression patterns of let-7 microRNA in the silkworm (Bombyx mori )Analysis of the structure and expression of the 30K protein genes in silkworm, Bombyx moriPurification, characterization and cloning of a chymotrypsin inhibitor (CI-9) from the hemolymph of the silkworm, Bombyx moriIdentification of three annexin IX isoforms generated by alternative splicing of the carboxyl-terminal exon in silkworm, Bombyx moriAnalysis of a Novel Transcription Mode of Fhx/P25 Gene in Bombyx moriIdentification and expression pattern of Bmlark, a homolog of the Drosophila gene lark in Bombyx moriMolecular cloning and characterization of Bombyx mori sterol carrier protein x/sterol carrier protein 2 (SCPx/SCP2) geneIn silico identification of silkworm selenoproteomicsIdentification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx moriSequence structure and expression pattern of a novel anionic defensin-like gene from silkworm (Bombyx mori)昆虫性别决定的分子机制研究进展家蚕sans fille基因的分子克隆及序列分析家蚕血液胰凝乳蛋白酶抑制剂的多态性分布一个新的家蚕Kunitz类型CI基因的克隆和序列分析家蚕卵黄原蛋白及其受体基因家蚕组织蛋白酶D基因的克隆、序列分析及其表达谱研究中国野桑蚕性信息素合成激活肽(PBAN)基因的克隆及序列分析家蚕信息素结合蛋白BmPBP2和BmPBP3基因的初步鉴定及表达分析
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